Question 1

What routine maintenance is recommended for the Qubit 4 Fluorometer, with WIFI (Cat. No. Q33238)?

Accepted Answer

The Qubit 4 Fluorometer, with WIFI (Cat. No. Q33238), does not require any specific routine maintenance. However, based on customer experience, we recommend the following:

From time to time, it may be useful to check the opening of the Qubit for dust particles and dirt. If needed, you can use a lint-free cloth or lenses cleaning paper towels to wipe the inside of the Qubit with some 75%-95% ethanol.

If you experience inaccurate measurements, regardless of the kit you use, we recommend testing the light source of the instrument with the Qubit 4 System Verification Assay Kit (Cat. No. Q33238).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Question 2

I cannot find the Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Q33266) assay file to download to my Qubit instrument. Where can I find the necessary file?

Accepted Answer

To access the necessary file for the Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits, update the Qubit 4 firmware using this link. The newest firmware contains the required assay file.

Question 3

Does the Qubit Flex NGS Starter Kit (Cat. No. Q45893) include the Qubit Flex System Verification Assay Kit (Cat. No. Q33254)?

Accepted Answer

No. The Qubit Flex NGS Starter Kit (Cat. No. Q45893) does not include the Qubit Flex System Verification Assay Kit (Cat. No. Q33254). It must be purchased separately.

The description of the Qubit Flex Starter Packages (Cat. Nos. Q32894, Q45893, Q45894) can be found on the Qubit Flex Starter Packages product page.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Question 4

Does the Qubit Flex Quantitation Starter Kit (Cat. No. Q45894) include a Qubit dsDNA Broad Range Assay Kit (Cat. No. Q33265, Q33266)?

Accepted Answer

No. The Qubit Flex Quantitation Starter Kit (Cat. No. Q45894) does not include a Qubit dsDNA Broad Range Assay Kit (Cat. No. Q33265, Q33266). It must be purchased separately.

The description of the Qubit Flex Starter Packages (Cat. Nos. Q32894, Q45893, Q45894) can be found on the Qubit Flex Starter Packages product page.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Question 5

Can I use the Qubit Endotoxin Detection Assay Kit on the Qubit 4 Fluorometer?

Accepted Answer

No, the Qubit Endotoxin Detection Assay Kit is designed for use with the Qubit Flex Fluorometer and is not currently for use on the Qubit 4 Fluorometer.

Question 6

To use the Qubit Endotoxin Detection Assay Kit on the Qubit Flex Fluorometer, do I need to perform a software update ?

Accepted Answer

Yes, the endotoxin detection assay program will be available on Qubit Flex Software version 1.8.0 or later. The latest software is available under the Resources section of www.thermofisher.com/qubit

Question 7

How do I store the Qubit Endotoxin Detection Assay Kit?

Accepted Answer

The entire kit can be stored at 2-8 degrees C for 6 months. Reconstituted Endotoxin standard solutions are stable for 4 weeks at 2-8 degrees C and reconstituted dye can be stored for 4 weeks at 2-8 degrees C. Reconstituted amebocyte lysate solution is stable for 1 week at -20 degrees C or colder if frozen immediately after reconstitution.

Question 8

Is the Qubit Flex Fluorometer (Cat. No. Q33327) compatible with custom assays?

Accepted Answer

No, the Qubit Flex Fluorometer does not have custom assay capability.

Question 9

The settings options on my Qubit instrument are greyed out and I am being prompted to sign in. How can I resolve this?

Accepted Answer

Our Qubit 4 and Qubit Flex instruments will now require users to sign in to access certain menu options (i.e., instrument settings, software update, and system verification). This is necessary to adhere to cybersecurity legal standards that were put in place in 2020. Only when signed in, are all functions of the device are accessible.

Instructions for login:
- Turn on the device
- From the home screen, touch the person icon on the top left corner
- Touch the "Get Started" button
- Touch the "Create Profile" button
- Enter information for username and pin
- Log in by touching the person icon and entering the user info in the sign in fields

Question 10

Which is the best DNA or RNA quantification instrument?

Accepted Answer

All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Question 11

What is the difference between UV and fluorescence DNA quantification? Which DNA quantification technology should I choose?

Accepted Answer

UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Question 12

When using the Qubit Flex Fluorometer, do I have to use all 8 samples/wells?

Accepted Answer

No, the Qubit Flex Fluorometer allows you to select which wells will be used for the experiment. The standards and sample will only be read in the selected wells. If the option to select wells is not available on your Qubit Flex, please update to the current firmware version (download firmware from Technical Resources for Qubit Fluorometers). This feature was introduced in version 1.5.0.

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