Question 1

What is the difference between UV and fluorescence DNA quantification? Which DNA quantification technology should I choose?

Accepted Answer

UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Question 2

Which is the best DNA or RNA quantification instrument?

Accepted Answer

All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Question 3

What is the difference between UV and fluorescence DNA quantification? Which DNA quantification technology should I choose?

Accepted Answer

UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Question 4

Micro-LC 产品 在广东或广州可以咨询报价吗？

Accepted Answer

可以。森德仪器可围绕 Micro-LC 产品 提供广东、广州及华南区域的选型咨询、配置确认、报价沟通、采购支持、安装培训和售后协同服务。

Question 5

Micro-LC 产品 适合哪些应用场景？

Accepted Answer

Micro-LC 产品 适合关注 低流速 LC-MS、微量样本、蛋白质组学和提高质谱联用灵敏度的液相分离 的实验室。实际配置需要结合样品类型、检测限、通量、软件和安装条件确认。

Question 6

Micro-LC 产品 询价前需要准备哪些信息？

Accepted Answer

建议准备检测项目、样品基质、目标化合物、检测限或定量限、每天样品量、现有液相条件、软件合规要求、预算范围和安装城市。

Question 7

Micro-LC 产品 是否适合第三方检测机构？

Accepted Answer

如果检测机构关注方法稳定性、样品通量、数据可追溯和售后响应，Micro-LC 产品 可纳入选型评估，具体要按检测项目和方法包确认。

Question 8

Micro-LC 产品 是否适合高校和科研平台？

Accepted Answer

Micro-LC 产品 可结合科研平台的课题方向、样品复杂度、定性/定量需求和共享平台管理方式评估，重点看灵敏度、分辨率、扫描速度和软件工作流。

Question 9

Micro-LC 产品 和相邻型号如何选择？

Accepted Answer

建议从检测目标、灵敏度、分辨率、扫描模式、通量、预算和未来扩展需求比较，而不是只看型号名称。必要时可把样品和目标方法交给工程师做配置建议。

Question 10

Micro-LC 产品 是否需要搭配液相色谱系统？

Accepted Answer

多数 LC-MS/MS 或 QTOF 工作流需要液相色谱配合。选型时应同步确认流速范围、色谱柱、进样量、梯度方法、样品前处理和接口兼容性。

Question 11

Micro-LC 产品 的安装条件要注意什么？

Accepted Answer

需要确认实验室空间、电源、气源、排风、温湿度、网络、数据工作站、耗材存放和维护通道，最终以厂家交付条件为准。

Question 12

Micro-LC 产品 是否可以用于方法开发？

Accepted Answer

可以作为方法开发的候选平台。方法开发要同时评估离子源、扫描方式、色谱条件、样品前处理、定量范围、质控策略和数据处理软件。

常见问题

What is the difference between UV and fluorescence DNA quantification? Which DNA quantification technology should I choose?

Which is the best DNA or RNA quantification instrument?

What is the difference between UV and fluorescence DNA quantification? Which DNA quantification technology should I choose?

Micro-LC 产品 在广东或广州可以咨询报价吗？

Micro-LC 产品 适合哪些应用场景？

Micro-LC 产品 询价前需要准备哪些信息？

Micro-LC 产品 是否适合第三方检测机构？

Micro-LC 产品 是否适合高校和科研平台？

Micro-LC 产品 和相邻型号如何选择？

Micro-LC 产品 是否需要搭配液相色谱系统？

Micro-LC 产品 的安装条件要注意什么？

Micro-LC 产品 是否可以用于方法开发？