NanoDrop™ 3300 荧光分光光度计
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此型号的新产品已经停产。如果您将在工作流程中使用荧光技术,请考虑使用 Invitrogen™ Qubit™ 4 荧光计 实现准确、特异性、灵敏、简单和快速的定量。 对于微量 UV-Vis 技术的较新进展, Thermo Scientific™ NanoDrop™ One/One C 型号 提供增强的功能(例如,区分核酸样品中的哺乳动物 DNA 和 RNA)以及支持在符合 21 CFR 第11部分规定的实验室中进行合规使用的软件。
此型号的新产品已经停产。如果您将在工作流程中使用荧光技术,请考虑使用 Invitrogen™ Qubit™ 4 荧光计实现准确、特异性、灵敏、简单和快速的定量。对于微量 UV-Vis 技术的较新进展,Thermo Scientific™ NanoDrop™ One/OneC 型号提供增强的功能(例如,区分核酸样品中的哺乳动物 DNA 和 RNA)以及支持在符合 21 CFR 第11部分规定的实验室中进行合规使用的软件。
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专业版本的 Microsoft™ Windows™ 7(32位和64位)、专业版本的 Windows 8.1(64位)、Windows 10 Pro(64位)
采购问答
<p>The NanoDrop 3300 is routinely used for the following: <br/> - Determine the concentration of nucleic acids (some of the pre-configured methods are dsDNA Hoechst dye, dsDNA PicoGreen dye and RNA RiboGreen dye) <br/> - Characterize molecular beacon probes labeled with different fluorophores <br/> - Assess the fluorescence of dyes such as Alexa, Cy and DyLightdyes <br/> - Measure fluorescence of a wide variety of inherent fluorophores</p>
<p>Yes, we recommend using dry laboratory wipes as a very effective method for removing the sample. The highly polished quartz and stainless-steel surfaces of the sample retention system are resistant to sample adherence. </p>
<p>Using a 2 µL sample, the range using the Quant-iT protein assay is 0.01 µg - 1.0 µg (BSA). <br/> The range using the fluorescein assay is > 4 logs. <br/> Detailed performance data using other fluorophores may be found on the Thermo Fisher website.</p>
<p>The NanoDrop 3300 Fluorospectrometer is very affordable compared to high-end fluorescence instruments, primarily because the NanoDrop 3300 Fluorospectrometer does not use costly monochromators.</p>
<p>The Fluorescence Profiler feature, in the NanoDrop 3300 Fluorospectrometer, allows LED excitation selection guidance when you are unsure of the excitation source and/or emission profile of a specific or unknown fluorophore.</p>
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<p>The NanoDrop 3300 Fluorospectrometer system is very user-friendly, even for those with limited fluorescence experience. There are no filter changes and the software is designed to make establishing and saving a standard curve quick and easy. Measuring a sample is as simple as pipetting a 2 µL sample onto the sample pedestal and clicking a button on the computer screen to initiate the measurement.</p>
<p>We recommend using a 2 µL sample size for protein measurements using a NanoDrop 3300 Fluorospectrometer. Proteins and/or protein buffers may alter the surface tension properties of the solution and using the larger sample size is recommended to ensure proper column formation.</p>
<p>The compact NanoDrop 3300 Fluorospectrometer allows for highly sensitive protein concentration measurements. Coupled with the fact that only a very small sample size is needed, this is a unique, accurate and quick (2-10 sec) way to make measurements when only limited (ultra-low) sample mass is available for testing.</p>
<p>Yes, for measuring dye-labeled proteins, pre-configured methods on the NanoDrop 3300 include: <br/> - Quant-iT Protein <br/> - Fluorescamine <br/> - FluoroProfile <br/> - Fluoraldehyde-OPA <br/> In addition, the broad range of excitation wavelengths and broad range in detecting emission wavelengths allow the flexibility of setting up custom methods using virtually any fluorophore (400-750 nm).</p>
<p>The NanoDrop 3300 does not require calibration. The NanoDrop 3300 bases all quantitative measurements on a standard curve so pathlength calibration is not required. As a result, there is no calibration solution for the NanoDrop 3300. Instead, a user may measure a serial dilution of a fluorescent dye such as fluorescein to assess reproducibility and linearity. <br/> There is a source check available on the main menu to confirm that the hardware is functioning properly.</p>