兼容： 专业版本的 Microsoft™ Windows™ 7（32位和64位）、专业版本的 Windows 8.1（64位）、Windows 10 Pro（64位）
采购问答
NanoDrop™ 3300 荧光分光光度计 常见问题
What additional analytes or biomolecules are routinely analyzed using the NanoDrop 3300 Fluorospectrometer?
<p>The NanoDrop 3300 is routinely used for the following: <br/>
- Determine the concentration of nucleic acids (some of the pre-configured methods are dsDNA Hoechst dye, dsDNA PicoGreen dye and RNA RiboGreen dye) <br/>
- Characterize molecular beacon probes labeled with different fluorophores <br/>
- Assess the fluorescence of dyes such as Alexa, Cy and DyLightdyes <br/>
- Measure fluorescence of a wide variety of inherent fluorophores</p>
Can I simply wipe the pedestal surface, of the NanoDrop 3300 Fluorospectrometer, enough to prevent carryover?
<p>Yes, we recommend using dry laboratory wipes as a very effective method for removing the sample. The highly polished quartz and stainless-steel surfaces of the sample retention system are resistant to sample adherence. </p>
What is the dynamic range for proteins that can be measured on the NanoDrop 3300 Fluorospectrometer?
<p>Using a 2 µL sample, the range using the Quant-iT protein assay is 0.01 µg - 1.0 µg (BSA). <br/>
The range using the fluorescein assay is > 4 logs. <br/>
Detailed performance data using other fluorophores may be found on the Thermo Fisher website.</p>
Is the NanoDrop 3300 Fluorospectrometer affordable, even though it allows for a large amount of flexibility?
<p>The NanoDrop 3300 Fluorospectrometer is very affordable compared to high-end fluorescence instruments, primarily because the NanoDrop 3300 Fluorospectrometer does not use costly monochromators.</p>
Can I use the NanoDrop 3300 Fluorospectrometer if I do not know what excitation source to use for my sample?
<p>The Fluorescence Profiler feature, in the NanoDrop 3300 Fluorospectrometer, allows LED excitation selection guidance when you are unsure of the excitation source and/or emission profile of a specific or unknown fluorophore.</p>
I have no previous experience with fluorescence techniques. Is the NanoDrop 3300 Fluorospectrometer easy to use?
<p>The NanoDrop 3300 Fluorospectrometer system is very user-friendly, even for those with limited fluorescence experience. There are no filter changes and the software is designed to make establishing and saving a standard curve quick and easy. Measuring a sample is as simple as pipetting a 2 µL sample onto the sample pedestal and clicking a button on the computer screen to initiate the measurement.</p>
What are the sample size requirements for my NanoDrop 3300 Fluorospectrometer?
<p>We recommend using a 2 µL sample size for protein measurements using a NanoDrop 3300 Fluorospectrometer. Proteins and/or protein buffers may alter the surface tension properties of the solution and using the larger sample size is recommended to ensure proper column formation.</p>
What are the advantages of using the NanoDrop 3300 Fluorospectrometer?
<p>The compact NanoDrop 3300 Fluorospectrometer allows for highly sensitive protein concentration measurements. Coupled with the fact that only a very small sample size is needed, this is a unique, accurate and quick (2-10 sec) way to make measurements when only limited (ultra-low) sample mass is available for testing.</p>
Can I quantify proteins using my NanoDrop 3300 Fluorospectrometer?
<p>Yes, for measuring dye-labeled proteins, pre-configured methods on the NanoDrop 3300 include: <br/>
- Quant-iT Protein <br/>
- Fluorescamine <br/>
- FluoroProfile <br/>
- Fluoraldehyde-OPA <br/>
In addition, the broad range of excitation wavelengths and broad range in detecting emission wavelengths allow the flexibility of setting up custom methods using virtually any fluorophore (400-750 nm).</p>
Can I calibrate my NanoDrop 3300 Fluorospectrometer?
<p>The NanoDrop 3300 does not require calibration. The NanoDrop 3300 bases all quantitative measurements on a standard curve so pathlength calibration is not required. As a result, there is no calibration solution for the NanoDrop 3300. Instead, a user may measure a serial dilution of a fluorescent dye such as fluorescein to assess reproducibility and linearity. <br/>
There is a source check available on the main menu to confirm that the hardware is functioning properly.</p>
