Orbitrap™ IQ-X™ Tribrid™ 质谱仪

<p>Watch this <a href="https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/mass-spectrometry-support-center/liquid-chromatography-mass-spectrometry-instruments-support/orbitrap-id-x-tribrid-mass-spectrometer-support/orbitrap-id-x-tribrid-mass-spectrometer-support-getting-started.html?open=g8#g8">video</a>.<br/><br/>Find additional tips, troubleshooting help, and resources within our <a href="https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/mass-spectrometry-support-center.html?icid=faqsc39">Mass Spectrometry Support Center</a>.</p>

<p>To achieve in-depth MSⁿ analysis, this method exhaustively interrogates a precursor using a combination of fragmentation mechanisms, MSⁿ levels, and m/z analyzers. At high MSⁿ levels, the product ion signals can become quite weak. To address this concern, we developed an “MSⁿ Quality Trigger”, which enables the user to trigger complementary ITMSⁿ scans if the corresponding FTMSⁿ scan S/N drops too low. Mass Frontier 8.0 software can curate “MSⁿ library” data into MSⁿ “tree” libraries, and the software can search unknown MSⁿ data against this local library.<br/><br/>Find additional tips, troubleshooting help, and resources within our <a href="https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/mass-spectrometry-support-center.html?icid=faqsc39">Mass Spectrometry Support Center</a>.</p>

<p>The assisted collision energy acquisition strategy uses a series of hidden ion trap scans to determine the optimal CE to use in the subsequent analytical scan. These IT spectra only scan a small mass range around the precursor ion (2 Th) to increase the analysis speed. The TIC of these IT scans is compared to the TIC of an IT scan of 0 collision energy to produce a pseudo-breakdown curve of the precursor. Once the amount of unreacted precursor crosses a user-defined threshold (e.g., 20%), the analytical MS/MS scan uses that 'optimal' collision energy. View images and learn more <a href="https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/mass-spectrometry-support-center/liquid-chromatography-mass-spectrometry-instruments-support/orbitrap-id-x-tribrid-mass-spectrometer-support/orbitrap-id-x-tribrid-mass-spectrometer-support-getting-started.html?open=g6#g6">here</a>.<br/><br/>Find additional tips, troubleshooting help, and resources within our <a href="https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/mass-spectrometry-support-center.html?icid=faqsc39">Mass Spectrometry Support Center</a>.</p>

<p>Assisted collision energy is now an available feature on all Tribrid instruments, software version 3.1. Through the Method Editor GUI, users can select different 'Collision Energy Modes' for both HCD and CID activation types in the 'Scan Properties' panel. View images and learn more <a href="https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/mass-spectrometry-support-center/liquid-chromatography-mass-spectrometry-instruments-support/orbitrap-id-x-tribrid-mass-spectrometer-support/orbitrap-id-x-tribrid-mass-spectrometer-support-getting-started.html?open=g5#g5">here</a>.<br/><br/>Find additional tips, troubleshooting help, and resources within our <a href="https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/mass-spectrometry-support-center.html?icid=faqsc39">Mass Spectrometry Support Center</a>.</p>

<p>Internal calibrant (reference) ions are used for real-time fine adjustment of the instrument's mass-to-charge ratio calibration, enabling a correction for otherwise uncompensated errors that are due to temperature changes scan-to-scan. The purpose of the internal calibration is to ensure high mass measurement accuracy, regardless of the MSⁿ level. This is especially important for low m/z ions over extended periods of time.<br/><br/>Find additional tips, troubleshooting help, and resources within our <a href="https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/mass-spectrometry-support-center.html?icid=faqsc39">Mass Spectrometry Support Center</a>.</p>

<p>The three modes offered are:<br/><br/> 'Background exclusion workflow'<br/> The background exclusion workflow allows users to generate DDMSⁿ data with automatic application of instrument generated background exclusion list. This allows detection of low level analytes in the presence of a complex background.<br/><br/> 'Background exclusion and component inclusion workflow'<br/> The background exclusion and component inclusion workflow allows DDMSⁿ data generation with automatic application of instrument generated inclusion and exclusion list. Multiple samples can be submitted in one sequence for their respective inclusion and exclusion list generation followed by one identification experiment using those lists.<br/><br/> 'Deep scan workflow' <br/> The deep scan workflow allows the generation of DDMSⁿ data with automatic application of inclusion and exclusion lists followed by automated re-injection of the sample. The inclusion and exclusion lists are updated prior to re-injection by moving triggered targets from the inclusion list to the exclusion list allowing deeper interrogation of complex samples.<br/><br/>Find additional tips, troubleshooting help, and resources within our <a href="https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/mass-spectrometry-support-center.html?icid=faqsc39">Mass Spectrometry Support Center</a>.</p>